经典Adams谱序列E₂项的相关结论是探究π_?S的关键要素.利用May谱序列以及对相应次数和微分的分析,给出了经典 Adams 谱序列 E₂ 项中元素族 b₁ k₀δ? _s+4 ∈ Ext_A^s+8,t(Z_p ,Z_p)的非平凡性,其中,p ≥ 11,0< s< p-4, t = q [(s+ 4) p³ +(s+ 4) p² +(s+ 4) p+ (s+ 2) ]+s.

基于矩匹配技术,研究价差期权定价问题.通过矩匹配技术利用逐段几何布朗运动逼近资产组合,进而推导出Vasiek-Ornstein-Uhlenbeck模型下看涨价差期权和看跌价差期权定价公式.最后,将基于蒙特卡罗模拟技术和矩匹配技术下的两种期权价格进行比较研究,结果表明在保持计算准确性的前提下,后者的计算效率远远高于前者.

利用时域有限差分(FDTD)方法,计算了由金纳米球和金纳米棒构成的核-卫星结构二聚体的局域表面等离子体共振(LSPR)特性.研究了金纳米球对金纳米棒耦合作用的影响.模拟出的散射光谱表明,在金纳米球的影响下,核-卫星结构二聚体的传感性能要明显优于单个金纳米棒.研究了不同金纳米球半径与不同金纳米棒长径比下的核-卫星结构二聚体的传感性能,总结出核-卫星结构二聚体的最优组装尺寸.结合偶极子共振模型和纳米颗粒的极化作用,对核-卫星结构二聚体的传感性能变化做了初步分析.

为了有效估计在低信噪比条件下的跳频信号参数,提出了一种基于改进Wigner-Ville分布(modified Wigner-Ville distribution, MWVD)的估计算法,该算法通过选择一个低阶的切比雪夫多项式构造核函数以减小交叉项,然后,通过MWVD结果可以计算得到跳频信号的参数估计,仿真结果表明提出的算法可以有效估计跳频信号的参数,与其他算法相比,在低信噪比条件下该算法性能更优.

构建了一套透射式的纳米等离激元暗场显微系统,光源入射端采用连续波长调制暗场光源的技术方法,探测端采用灰度工业相机连续采集对应波长的暗场图像,利用自编的matlab程序对图像进行光谱提取,提高了纳米粒子的散射光谱的检测精度.采集纳米粒子聚集前后的局域表面等离子共振光谱,实验结果与边界元方法(BEM)仿真结果一致.

传统断面检测器存在检测范围有效、完好率不足等实际问题无法有效感知和辨识路网交通状态,以浮动车数据为基础,研究和设计了路网交通宏微观评价指标,并提出基于灰色关联熵的路网宏微观交通状态评价方法,进一步通过引入宏观基本图平移的特性扩展了交通状态评价方法.最后,利用北京市昌平区政府街主干线数据对所提方法进行了验证,结果表明所提方法能够有效判别路网的宏微观交通状态.

为了探究急性缺血性脑卒中(Acute ischemic stroke, AIS)患者和对照组静脉血血清中的O-GlcNAc糖蛋白差异,筛选AIS诊断的潜在生物标志物.首先对AIS和对照组血清中的O-GlcNAc糖基化修饰进行检测,发现AIS的O-GlcNAc修饰程度明显高于对照组(P<0.05),通过CpOGAD298N富集AIS和对照组血清中的OGlcNAc蛋白并进行质谱鉴定,得到差异蛋白肝素辅助因子II(Heparin Cofactor II, HC2),Western blot检测结果表明,富集蛋白中HC2丰度在AIS组显著高于对照组(P<0.01),ROC曲线分析显示,HC2对急性缺血性脑卒中具有潜在的诊断价值(AUC=0.882),并且高于常用的血脂指示标志.

脊髓损伤(SCI)是一种致命的中枢神经系统创伤,目前尚无有效修复手段.肉苁蓉提取物可以治疗脊髓损伤,但是,作用机制仍然不清楚.运用网络药理学和分子对接技术探讨肉苁蓉治疗SCI的分子机制.肉苁蓉的活性成分和靶点由中药系统药理学数据库和分析平台(TCMSP)预测.SCI相关的靶点从GeneCards、Drug Bank、PharmGkb和OMIM数据库中获得,交集靶点由Venny2.1.0获得.通过String数据库和Cytoscape软件绘制了蛋白质相互作用(PPI)网络.接下来,利用Bioconductor平台进行KEGG通路分析.使用Autodock Vina进行分子对接.根据筛选标准,从肉苁蓉中得到6个活性成分和85个潜在靶基因.通过在线数据库,得到6 737个SCI相关靶点和肉苁蓉与SCI的74个交集靶点.在活性成分靶点网络中,β-谷甾醇、槲皮素和花生四烯酸是重要的活性成分.在PPI网络中,HIF1A、FOS、AR、RELA、EGFR和CCD1等是核心靶点.KEGG分析表明肉苁蓉治疗SCI的主要途径涉及PI3K-Akt、TNF和MAPK等信号通路.在分子对接中,肉苁蓉的活性化合物槲皮素与核心靶点具有良好的的亲和力.最后,体外实验验证表明,肉苁蓉对SCI的治疗可能主要是通过其活性成分槲皮素抑制炎症和氧化反应,从而改善SCI微环境.为进一步肉苁蓉治疗SCI的临床应用提供了新思路.

基于Wnt/β-连环蛋白(β-catenin)信号通路探究壮医通痹千斤方对膝关节炎(KOA)大鼠改善作用.通过注射木瓜蛋白酶方法建立KOA大鼠模型,随机分为模型组、治疗组、治疗组+激活剂(氯化锂)组和阳性对照组(塞来昔布胶囊),并以注射等体积的生理盐水作为对照组;收集大鼠外周血及软骨组织,检测各组大鼠血清中白细胞介素(IL)-6和肿瘤坏死因子-α(TNF-α)水平;观察软骨组织显微结构、病理学变化及损伤评分;分析软骨组织中Wnt-3a、β-catenin、runt相关转录因子2(Runx2) mRNA及蛋白表达.与对照组相比,模型组软骨细胞减少且零星分布、膜结构出现边缘空洞或空泡样,IL-6和TNF-α表达水平增加,Wnt-3a、β-catenin和Runx2 mRNA蛋白表达升高以及Mankin评分显著增加(P<0.05).与模型组相比,治疗组和阳性对照组软骨细胞明显增加,排列较为整齐、空泡样结构减少,IL-6和TNF-α表达水平下降、Wnt-3a、β-catenin和Runx2 mRNA蛋白表达降低以及Mankin评分显著降低(P<0.05);与治疗组相比,治疗组+激活剂组细胞排列紊乱,软骨细胞数目减少,空泡样结构增加,IL-6和TNF-α表达水平增加,Wnt-3a、β-catenin和Runx2 mRNA蛋白表达升高以及Mankin评分显著增加(P<0.05).结果表明:壮医通痹千斤方可以改善KOA大鼠损伤,可能与抑制Wnt/β-catenin信号通路有关.

The primary goal of this research is to investigate the clinical effect of transforaminal endoscopic spine system in the treatment of lumbar disc protrusion with vacuum phenomenon. A total of 124 patients with lumbar disc protrusion underwent transforaminal endoscopic spine system, including 85 patients with the non-vacuum phenomenon and 39 patients with the vacuum phenomenon, were retrospectively collected. VAS score,JOA score, improvement rate, and safety evaluation of patients with different signs were compared. Results showed that after the surgery, the VAS score decreased from (7.52±1.02) to (3.44±0.97) in vacuum phenomenon group (t=3.574 9, P=0.021), and from (7.35±0.99) to (2.51±0.85) in the non-vacuum phenomenon group (t=7.112 5, P<0.001). JOA score in the vacuum phenomenon group increased from (11.79±1.98) to (14.72±1.66)(t=2.075 8, P=0.195) in three monthes, and the non-vacuum phenomenon group increased from (12.21±2.01) to (19.25±1.57) (t=9.774 5, P<0.001). Postoperative C-reactive protein level, intraoperative blood loss, 24 h postoperative drainage volume, complication rate, and recurrence rate were all higher in vacuum phenomenon group. In conclusion, the transforaminal endoscopic spine system can effectively treat lumbar disc protrusion, but for patients with the vacuum phenomena, there may be long-term lumbar pain. Molecular dynamics simulations between IGFBP6 and IGF were performed, which gives us more information for the investigation of the pathogeny of the lumbar disc protrusion.

Cervical cancer (CC) is a common malignant gynecologic tumor that is infected by the human papillomavirus (HPV). Astragalus also known as Huangqi in Chinese can enhance curative efficacy and reduce chemotherapy toxicity for cervical cancer. However, the action mechanism remains unclear. The network pharmacology, molecular docking, GEPIA, and in vitro experiments were used to explore the underlying molecular mechanism of Huangqi in treating CC. Results showed that a total of 20 active compounds and 180 potential targets of Huangqi were chosen. In an drug-active component-target-disease network, quercetin, kaempferol, formononetin, and isorhamnetin were the important active components. In PPI network, MAPK1, AKT1, CCND1, FOS, JUN, MAPK14, RELA, HIF1A, IL-2, MYC, and ESR1 were the core targets. The expression of ESR1 was significantly lower in tumor tissues than in normal control tissues by GEPIA analysis. KEGG analyses showed that the main pathways of Huangqi in treating CC involved TNF, MAPK, and PI3K-Akt signaling pathways. In molecular docking, the active compounds of Huangqi had a good affinity with ESR1. Finally,the MTT assay and qPCR analysis validated the therapeutic effect of the active ingredients of Huangqi injection on cervical cancer.

Renal clear cell carcinoma (ccRCC) is considered to be the most common renal cell carcinoma. Molecular targeted therapy and immunotherapy improve the prognosis of patients with advanced RCC, but recurrence, progression of distant metastasis, and side effects are still important issues related to these treatments. Syndecans is a multi-functional family including four cell surface molecule members, which has various biological functions. Syndecan-3 (SDC3) is the largest one, which not only can affect a variety of physiological functions but also affect the progression of many cancers. However, its expression in renal cell carcinoma and its possible role in the progression and metastasis of renal cell carcinoma is not clear. According to the TCGA database and IHC analysis, the mRNA and protein levels of SDC3 were significantly increased in ccRCC tissues were found. The expression of SDC3 was associated with the prognosis of patients with renal cell carcinoma and with the tumor size of the patients was found. Further research indicated that SDC3 knockdown inhibits the proliferation of ccRCC cell lines. In summary, our study demonstrated high expression of SDC3 promotes ccRCC cell malignancy and sheds light on developing novel therapeutic approaches based on SDC3.

基于网络药理学和分子生物学探讨白花蛇舌草对人胶质瘤U251细胞生长及细胞周期分布的影响及机制.应用CCK-8试剂盒、划痕实验和流式细胞术检测白花蛇舌草抗脑胶质瘤的作用;筛选白花蛇舌草抗肿瘤活性成分,应用网络药理学方法构建白花蛇舌草活性成分-作用靶点-疾病的相互作用网络并应用分子生物学手段解析其作用机制.研究发现,白花蛇舌草可抑制U251细胞的增殖和迁移,诱导该细胞的凋亡,阻止该细胞的周期进展从而发挥其抗脑胶质瘤作用.其作用机制可能是多种抗肿瘤活性成分通过多个信号通路影响细胞功能,其中熊果酸可抑制U251细胞的增殖并诱导细胞凋亡,伴有Bax/Bcl-2表达水平增加.为进一步研究白花蛇舌草抑制胶质瘤的药效物质基础提供了理论依据.

以稻壳为原材料对染料废水中的亚甲基蓝进行吸附研究.考察了稻壳粒径、吸附时间、吸附剂用量、pH和初始浓度对染料废水的吸附效果，并对其进行了动力学研究.结果表明，最佳吸附条件为:初始浓度为100mg·L^-1 ,吸附剂用量为0.125g，吸附时间为2h，pH=6.动力学研究表明，稻壳对亚甲基蓝的吸附符合准二级动力学方程，其平衡吸附量达到32.787 mg·g^-1.

针对松材线虫病的在森林传播会导致毁灭性病虫害问题，以松褐天牛在松材线虫传播过程中的媒介作用为基础，构建了基于饱和发生率、人工防治延迟时滞等非线性变化特征的生态侵染模型.模型将媒介昆虫分为易感天牛和染病天牛，通过分析饱和发生率、人工防治时滞对生态系统稳定性的影响，模拟求解并预测松材线虫病对森林生态侵染的发生趋势.案例研究的结果表明：当人工防治不存在时间延迟（τ=0）时，天牛种群将在一段时间后达到稳态，病害不会发生大规模爆发；当人工防治力度低于24%左右时，易感天牛和染病天牛的种群数量随防治呈上升趋势，无法达到防治效果；当人工防治力度超过24%左右时，防治力度越强，染病天牛的种群数量越少且减小速率越快，可以达到防治效果；人工防治延迟的临界时滞为16 d.

β多样性是生物多样性的重要组成, 描述不同群落间物种组成差异, 由物种替换和丰富度差异两部分组成, 其形成机制也是恢复生态学的研究热点.以北大港湿地自然保护区植物群落为研究对象, 揭示其β多样性特征.结果表明：（1）β多样性样方贡献度低的样地集中于万亩鱼塘和沙井子水库, 植物群落特异度较低,物种较为均质单一.而北大港湿地核心区的群落组成特异度高, 植被恢复效果好, 应重点加强该区域的植被监测.（2）碱蓬（Suaeda glauca）、芦苇（Phragmites australis）、狗尾草（Setaria viridis）和盐地碱蓬（Suaeda salsa）变化会显著影响到β多样性现有格局, 是北大港湿地的重要生态指示种.（3）在物种替换和丰富度差异中, 北大港湿地植被β多样性以物种替换为主, 占比64.09%,而丰富度差异占比35.91%, 因此在制定植被多样性保护管理措施时, 需要重点保护多样性替换度高的区域.（4）物种替换与物种丰富度、Shannon熵指数、Pielou均匀度指数、Shannon均匀度指数和Simpson均匀度指数呈显著负相关.